kudzuvines: (Default)
Method, a company that makes eco-friendly cleaning products, don't care that you think advertisements depicting sexual harassment in a woman's own home are inappropriate and downright disgusting.


Read it, get angry, send letters, don't buy their products, and tell others not to, either.

I mean, I have a pretty dirty, cynical sense of humor, but this ad isn't funny--it's creepy as hell and only made me want to avoid Method products in the future. Way to go, guys.

The most frustrating thing is that they probably think negative publicity is still publicity, and they'd be right--but they have seriously damaged their reputation.

I'm a feminist. You should be too. Radical notion that women are people and all that.
kudzuvines: (Default)
Interestingly, 1:48 appears to be when I post sadly about procrastination.
kudzuvines: (Default)
And then... she didn't update for the entire month of May! And almost June!

We're goin' on a roadtrip. We leave in a little less than a month. Many of you I will see, and need to talk to about this. It is a big road trip. Of big-itude.


Almost done with my job, which is excellent. I am muy sick of membrane proteins. I am never going to work with them again. Evar.

Um, my friends in Portland--some of them have started leaving for their own adventures, or are about to leave. Sad, we're all dispersing. But I'll find more people, just like I have in Portland. I'm sure of it. I've already started talking to my future classmates in Santa Cruz.

Hopin' my coworker can take the bunnies. Or bunny, if we end up getting rid of Robert Smith of the Cure. He and Pants do not get along.

Want to go hiking again before I leave...
kudzuvines: (Default)
Rant time!

There is a certain person in my lab (who shall remain nameless; those of you who know, will know) who has repeatedly gone over my head when it comes to the mouse colony. I manage the mouse colony. That is initially what I was hired to do. This involves basic things like breeding the mice, genotyping them, and keeping close track of which ones are needed for experiments. It's not a hard job, but it is made infinitely more difficult when people ignore that I am in charge of the colony and sacrifice mice without letting me know that that is what they are doing.

This has come to a head. He doesn't like the way we are going to be doing things in the future; sacrificing the mice in pairs (two people, not two mice), weighing the mice, weighing organs, etc. He likes working alone, he doesn't like coordinating with other people, because he likes to do things whenever it's completely convenient for him. When we told him on Friday that we were going to start doing standardized sacrifices, he complained. This morning I went down to the colony and noticed that three cages were missing. Since I am in charge of keeping track of things, I worried about it. I called him, knowing he was the most likely culprit, and he said he'd sacrificed them this weekend but hadn't emailed me yet.

What the fuck. I have told him repeatedly that he needs to inform me of anything he does with the colony. It's not like I'll tell him not to! I just need to fucking know!

I'm pretty sure he didn't tell me because he didn't want to "waste his time" doing the standardized procedures.

Fuck that. If he won't play nice, I'm not going to either. Now that I've written this entry, I'm going to go to talk to my boss. I'm tired of being disrespected over this.

[ED: This isn't the same problem as my last post.]
kudzuvines: (Default)
Today was the most excitingest comics day evar. Here's why:

-I met Allison Bechdel, bought her new book, heard her talk, and then she signed my book afterwards! She's pretty much the coolest published-author-comics-artist-writer-person ever, and she was super cute on stage--every time she wasn't sure of an answer or there was a pause, her mouth would twitch. Also, her favorite book that she'd have on a desert island if she couldn't have anything else would be Pride and Prejudice. How cool is that? And she said that while she started out with Mo as her avatar in Dykes to Watch Out For, Sidney has become her avatar because "well, I introduced her because I realized everyone in the comic was really pretty nice, and I wanted someone who wasn't, and well, I guess I'm not all that nice most of the time. Take from that what you will."

And then she signed my book and I awkwardly talked about Charis and how Sarah and I read all her books on the couches before HS Women's writer's group, and how I used to live in the Twin Cities and so did she. I imagine it must be kind of weird, having to share a Personal Moment with hundreds of people who want to meet you one after another...

-For the second bullet point, which is pretty much the only other one I have, I got to meet Erika Moen who writes Dar (http://www.webcomicsnation.com/erika/dar/series.php), which I love. We chatted for a long time and she was super cool and as awesome as she seems from her comic. I even watched her booth for five minutes when she had to go pee, and awkwardly managed to sort of sell the other guy at her table's comics. I'm pretty sure I was blushing the whole time I talked to her. Radcakes!

And so it was the best day for comix-awesome evar! Hurrah!
kudzuvines: (Default)
Read this article:


Call the number! Demand freedom!

ETA: Shit, guys, has everyone heard of the massive human rights abuses that are happening around the RNC? People have been under surveillance for months. Any left-wing group has possibly been infiltrated by citizens organized by the FBI to spy.

This is wrong. This is not being reported on.

Non-violent protesters are getting arrested for nothing. Preemptively.

Read this article too:


PROTEST. This is wrong.
kudzuvines: (Default)
[livejournal.com profile] sir_yessir tagged me in a meme. This must mean he wants to know all of my secrets.

Here goes.

Write three facts, two quirks, and two habits about you, then tag 8 people to do it too.

I bet this is the highlight of your day. )
kudzuvines: (Default)
Today sucks because:

1. Experiments are hard to do when you don't know what the next step should be because your PI is out of town.

2. My student only comes in in the afternoons, meaning I'm doing half her project for her.

3. I induced protein at unfortunately high levels of cells--the OD was waaay too high when I got around to the induction yesterday.

4. I didn't remember to add glucose, which meant they were growing crapily in the first place.

5. I came in this morning to find one liter of my cells had fallen over in the shaker and spilled all over the fucking thing--leaving both a mess and many fewer cells in which to induce protein.

6. The centrifuge had water sitting in the bottom of it.

7. The centrifuge rotor had enormous puddles of water in each of its wells.

8. I have no idea what fucking buffer to lyse my cell pellets in or do my column in because THERE ISN'T A PROTOCOL, I'm just looking things up on the internet. But there should be a protocol, since another lab has already done this. But that guy left. Leaving no protocol.

9. I have to use a French press again for the first time in 4 years (MK, I'm looking at you here... YOU WERE THERE in those horrible first days of French Pressy goodness). I HATE the French press. HATE.

10. I have to show my student how to use it. She will be confused and cutesy. I will have to refrain from hitting her or telling her to shut her fucking mouth already, her voice is giving me a headache.

11. Probably don't have nanodiscs after all. Will have to try again. Insect cells are not yet grown up after they died A MONTH AGO because someone is incompetent. I am so looking forward to September.
kudzuvines: (Default)
Hi Amanda!

So yesterday, I went into the darkroom, put my film down on my Western blots, and lo and behold...


And now, for the relentless proving, proving, proving, and also repetition. Must be repeatable, you see. But! RESULTS, YOU GUYS.


Jul. 15th, 2008 05:38 pm
kudzuvines: (Default)
I used math, my smarts, my biochemical skillz, and Problem Solving to figure out how much of my protein is present in my membrane prep! Huzzah.

Now I can make nanodiscs!
kudzuvines: (Default)
So I grabbed this meme from her.

"Everyone has things they blog about. Everyone has things they don't blog about. Challenge me out of my comfort zone by telling me something I don't blog about, but you'd like to hear about, and I'll write a post about it. Ask for anything: latest movie watched, last book read, political leanings, thoughts on yaoi, favorite type of underwear, life experiences etc. Repost in your own journal so that we can all learn more about each other."

Do it.


Mar. 4th, 2008 03:08 pm
kudzuvines: (Default)
Excited for Minnesota!

Somewhat worried about getting results before telling Boss-Lady that I will be away for a week... after being away for two days next week... yeaaah.

Um? We did the crossword puzzle in the park in the sun this Sunday. It was wonderful. Also, went skiing on Saturday--it was only the second time I've gone, but I tried some blue slopes with Peter. They were hard. He was patient and good at teaching me not to break my head. There was some ice and at one point I got horrible vertigo because it was snowing hard enough that everything was white and I couldn't tell if I was moving or not. Eugh. But the rest of it was fun!

Also, Peter gave me E. coli. No, my new plush sidekick.
kudzuvines: (Default)
The rules are easy, just post 10 things that recently made you happy! Then tag 7 people and force them to post this meme on their LJs. Because it is good. Everyone needs a little happiness once in a while.

ZOMG Happiness meme!!!! )
kudzuvines: (Default)
Fri, Mar 7, 2008
Depart: 05:45 PM
Arrive: 10:50 PM

Seattle/Tacoma, WA (SEA) to
Minneapolis, MN (MSP)Sun Country Airlines
Flight 234


Tue, Mar 11, 2008
Depart: 03:00 PM
Arrive: 04:30 PM

Minneapolis, MN (MSP) to
Seattle/Tacoma, WA (SEA)

(I'm taking the train from Portland to Seattle).

So! Those of you on my FL, take note! I will be in el Minnesote from March 7 to March 11! I want to see all of you who live there! YAY.

Part of my time there will be taken up by going to see Anna and Emily sing pants off of themselves, or at least Emily, because she plays a prostitute. The rest of my time is, as yet, unspoken for? I may, if I can get ahold of a car, go see my brother in Appleton, but this may not pan out.

Anyway! Back to regular scheduled things.

Tum te tum

Jan. 31st, 2008 04:13 pm
kudzuvines: (Default)
Have been sitting next to the Mass-Spec all day and now my hearing in one ear is all funky. Curse you, Mass Spec.

Science-babble )

Today I borrowed Peter's bike so that I can bike home from the climbing gym. Fuckin' bus. The super nice thing about a bike is that you don't rely on any kind of schedule--you just leave when you want and get home when you get home. No standing aimlessly in the cold, waiting for some driver to notice you in the dark. No creepy dudes at the bus stop. Bikedy bike. I really do need my own.

But that comes after tickets to Minnesota to see Anna and Emily sing their pants off (hopefully not, that might be embarrassing) and also having my bunny stripped of a uterus. It sounds so nice when you put it that way, doesn't it?

Also! I really need to clean up my room and get more lamps so it's not dark and cluttered and depressing. Indeed. It's still not completely clean from when I moved in. I might actually want to spend time there if it's clean and well-lit.

So yeah.
kudzuvines: (Default)
Hey kids, it's been a while.

Today I got E. coli guts on my hands. Mmm, delish.

Also, I found a gimptastic runty mouse from the immaculate conception litter (there's basically no way that the mama mouse could have HAD this litter). She's kind of pathetically amusing looking--her front half is too big for her back half, and she's about half the size of her littermates. She also stumbles around squinting. But she's managed to live this long... she might just make it to adulthood. She's probably horrifically inbred.

Now I'm waiting for an affinity column to drain. Yaaaay.

On Sunday I went mushroom hunting (edible), and we found lots of chanterelles. They cost a lot in the store, and it's fun to pick them, so we did.

Tomorrow I go to a bouldering gym with my new friend Sarah. We are excited! Yay!

Ok! Thrilling, I know.


Sep. 18th, 2007 05:42 pm
kudzuvines: (Default)
UGH. I just want to go home, but nooo, PCR took forever, gel is taking forever, I just want to GO HOME AND EAT. And nap. Or just go to bed. I'm tired and somewhat discouraged, since PCR hasn't been working for my and my boss essentially told me yesterday "Well, just keep doing it until it works. Change different things until it works for you!" instead of designing new primers. She's right, but I hate doing such repetitive things.

I have lots of mouse babies, though. They are incredibly cute and squinty, as only a few of their eyes have opened yet. I have 2 that are 2 weeks old today, 9 that are 12 days old (OMG SO CUTE), 4 that are 8 days old, and 6-ish that are only 3 days old or so. And another mouse is pregnant, and we're hoping that two other mice are pregnant. Yay mouse babies.

Tissue homogenization is one of those things that isn't too hard but would get awfully repetitive. I think I will avoid it as long as possible. However! I will get to play with an electron microscope next week.

That's all I feel like writing about work right now.

I want some sleep. Mmm, sleep.
kudzuvines: (Default)
... I wish I was in Minnesota, because then there'd be people I knew to hang out with and I wouldn't have to make an effort to get to know people.

I miss you guys.
kudzuvines: (Default)
Today, everyone, I got to watch a mouse give birth. I just happened to come downstairs right as this pregnant female started squeezing out her first pup. I set the cage down on the edge of the cage-holder-shelf and watched it all through the clear plastic bottom of the cage (the mice are given bedding, but in this particuar area, luckily, the mice had pushed it aside). It was quite fascinating; I'm not sure if everyone finds this sort of thing as fascinating as I do, but I hope so, because damn if birth isn't one of the weirdest, most amazing things that mammals do.

Herein lies mousey-birth details! )

In other news, circus classes start tomorrow!


kudzuvines: (Default)

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