kudzuvines: (Default)
Today sucks because:

1. Experiments are hard to do when you don't know what the next step should be because your PI is out of town.

2. My student only comes in in the afternoons, meaning I'm doing half her project for her.

3. I induced protein at unfortunately high levels of cells--the OD was waaay too high when I got around to the induction yesterday.

4. I didn't remember to add glucose, which meant they were growing crapily in the first place.

5. I came in this morning to find one liter of my cells had fallen over in the shaker and spilled all over the fucking thing--leaving both a mess and many fewer cells in which to induce protein.

6. The centrifuge had water sitting in the bottom of it.

7. The centrifuge rotor had enormous puddles of water in each of its wells.

8. I have no idea what fucking buffer to lyse my cell pellets in or do my column in because THERE ISN'T A PROTOCOL, I'm just looking things up on the internet. But there should be a protocol, since another lab has already done this. But that guy left. Leaving no protocol.

9. I have to use a French press again for the first time in 4 years (MK, I'm looking at you here... YOU WERE THERE in those horrible first days of French Pressy goodness). I HATE the French press. HATE.

10. I have to show my student how to use it. She will be confused and cutesy. I will have to refrain from hitting her or telling her to shut her fucking mouth already, her voice is giving me a headache.

11. Probably don't have nanodiscs after all. Will have to try again. Insect cells are not yet grown up after they died A MONTH AGO because someone is incompetent. I am so looking forward to September.
kudzuvines: (Default)
Hi Amanda!

So yesterday, I went into the darkroom, put my film down on my Western blots, and lo and behold...


And now, for the relentless proving, proving, proving, and also repetition. Must be repeatable, you see. But! RESULTS, YOU GUYS.


Jul. 15th, 2008 05:38 pm
kudzuvines: (Default)
I used math, my smarts, my biochemical skillz, and Problem Solving to figure out how much of my protein is present in my membrane prep! Huzzah.

Now I can make nanodiscs!

Tum te tum

Jan. 31st, 2008 04:13 pm
kudzuvines: (Default)
Have been sitting next to the Mass-Spec all day and now my hearing in one ear is all funky. Curse you, Mass Spec.

Science-babble )

Today I borrowed Peter's bike so that I can bike home from the climbing gym. Fuckin' bus. The super nice thing about a bike is that you don't rely on any kind of schedule--you just leave when you want and get home when you get home. No standing aimlessly in the cold, waiting for some driver to notice you in the dark. No creepy dudes at the bus stop. Bikedy bike. I really do need my own.

But that comes after tickets to Minnesota to see Anna and Emily sing their pants off (hopefully not, that might be embarrassing) and also having my bunny stripped of a uterus. It sounds so nice when you put it that way, doesn't it?

Also! I really need to clean up my room and get more lamps so it's not dark and cluttered and depressing. Indeed. It's still not completely clean from when I moved in. I might actually want to spend time there if it's clean and well-lit.

So yeah.


kudzuvines: (Default)

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