kudzuvines: (Default)
Today sucks because:

1. Experiments are hard to do when you don't know what the next step should be because your PI is out of town.

2. My student only comes in in the afternoons, meaning I'm doing half her project for her.

3. I induced protein at unfortunately high levels of cells--the OD was waaay too high when I got around to the induction yesterday.

4. I didn't remember to add glucose, which meant they were growing crapily in the first place.

5. I came in this morning to find one liter of my cells had fallen over in the shaker and spilled all over the fucking thing--leaving both a mess and many fewer cells in which to induce protein.

6. The centrifuge had water sitting in the bottom of it.

7. The centrifuge rotor had enormous puddles of water in each of its wells.

8. I have no idea what fucking buffer to lyse my cell pellets in or do my column in because THERE ISN'T A PROTOCOL, I'm just looking things up on the internet. But there should be a protocol, since another lab has already done this. But that guy left. Leaving no protocol.

9. I have to use a French press again for the first time in 4 years (MK, I'm looking at you here... YOU WERE THERE in those horrible first days of French Pressy goodness). I HATE the French press. HATE.

10. I have to show my student how to use it. She will be confused and cutesy. I will have to refrain from hitting her or telling her to shut her fucking mouth already, her voice is giving me a headache.

11. Probably don't have nanodiscs after all. Will have to try again. Insect cells are not yet grown up after they died A MONTH AGO because someone is incompetent. I am so looking forward to September.
kudzuvines: (Default)
Oh man. Today kinda sucked, for numerous tiny reasons. Let's begin at the beginning, shall we?

1. I woke up at 2:44. PM.

This doesn't sound like a problem to those of you who have to get up early, but I slept 12 hours and wasted soo much time... I was hoping to get up around 11 or noon, which is significantly more time.

2. No emails from anyone at all that I've been waiting for responses from.

This was just sad-making, and also irritable-making, as I want a job, kthxbai.

3. Non-existent block party.

On Friday I was walking around my neighborhood with someone and a neighbor asked if I lived in the area. "We're having a potluck next Tuesday! 6:30-8:30! You should come!" I got excited because I like block parties, and I like meeting neighbors, so I remembered and told my housemate and we made a delicious (cherries, blueberries, peaches, and canteloupe) fruit salad. We walked down the street to the park to find... no block party. Sad.

4. Friends are busy

We decided to take the fruit salad over to my friends Loren and Pamela's house so they could share with us and meet Katherine. I called, they actually picked up (they are notorious for never picking up), and said that for once, they were too busy to hang out. So we had nothing.

5. Safeway does not have cheesecloth.

I'm trying to make a dumpling. Okay? Nobody has cheesecloth. Fucknuggets. Or a vegetable steamer.

6. Stupid Religious Fat Lesbian has parked in the parking spot I had before I left 10 minutes ago to go to Safeway. My car will not be in the shade tomorrow.

7. I grow hungrier.

Everyone who knows me knows this is bad. I decided to make orzo with broccoli, kalamata olives, basil, and tomato. This, of course, requires orzo, broccoli, olives, and basil. Trader Joe's is closing in 8 minutes. I drive like a bat out of hell. I notice that the SRFL has left the parking lot again, and thus the space is open. I will get it when I return.

8. Trader Joe's also has no cheesecloth. And no basil. And no orzo.

But I did get the broccoli and olives. And some Mac 'n' Cheese.

9. Safeway has no basil.

You assholes. They were out. But I did get the tomato. And the orzo.

10. I return home and SRFL has come home and taken the goddamn spot again.


So I made the Mac 'n' Cheese. It was ok. Not as delicious as the orzo dish, though. Also, I had some fruit salad, and it's too bad those motherfuckers didn't have a potluck, because we made some delicious fruit salad. Oh well, more for us.

11. No one has emailed me, still.

No job, no friends. Boo. But I talked to Patricia. Yay!

Hopefully the next hour will be good. Then it will be tomorrow.


kudzuvines: (Default)

November 2009

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